Detailed information of TA system TA146291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3641145..3641839
• Replicon: chromosome
• Accession: NZ_CP047469
• Organism: Escherichia coli strain DY612

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GTK51_RS17780
• Protein ID: WP_001263493.1
• Coordinates: 3641145..3641543 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GTK51_RS17785
• Protein ID: WP_000554757.1
• Coordinates: 3641546..3641839 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3636805)	3636805..3636885	-	81	NuclAT_12	-	-
- (3636805)	3636805..3636885	-	81	NuclAT_12	-	-
- (3636805)	3636805..3636885	-	81	NuclAT_12	-	-
- (3636805)	3636805..3636885	-	81	NuclAT_12	-	-
GTK51_RS17750 (3636145)	3636145..3637389	-	1245	WP_000189541.1	esterase FrsA	-
GTK51_RS17755 (3637481)	3637481..3637939	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GTK51_RS17760 (3638200)	3638200..3639657	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
GTK51_RS17765 (3639714)	3639714..3640235	-	522	Protein_3471	peptide chain release factor H	-
GTK51_RS17770 (3640234)	3640234..3640437	-	204	Protein_3472	RNA ligase RtcB family protein	-
GTK51_RS17775 (3640683)	3640683..3641135	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GTK51_RS17780 (3641145)	3641145..3641543	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GTK51_RS17785 (3641546)	3641546..3641839	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GTK51_RS17790 (3641891)	3641891..3642946	-	1056	WP_063501914.1	DNA polymerase IV	-
GTK51_RS17795 (3643017)	3643017..3643802	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
GTK51_RS17800 (3643774)	3643774..3645486	+	1713	Protein_3478	flagellar biosynthesis protein FlhA	-
GTK51_RS17805 (3645710)	3645710..3646207	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T146291 WP_001263493.1 NZ_CP047469:c3641543-3641145 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T146291 NZ_CP062736:4211573-4211791 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT146291 WP_000554757.1 NZ_CP047469:c3641839-3641546 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT146291 NZ_CP062736:4211173-4211547 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6