Detailed information of TA system TA146273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 671676..672403
• Replicon: chromosome
• Accession: NZ_CP047469
• Organism: Escherichia coli strain DY612

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5P0J3S6
• Locus tag: GTK51_RS03325
• Protein ID: WP_063501682.1
• Coordinates: 671676..671990 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTK51_RS03330
• Protein ID: WP_000560266.1
• Coordinates: 671987..672403 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK51_RS03305 (667834)	667834..668820	-	987	WP_000617698.1	Gfo/Idh/MocA family oxidoreductase	-
GTK51_RS03310 (668899)	668899..669591	-	693	WP_063501683.1	vancomycin high temperature exclusion protein	-
GTK51_RS03315 (669668)	669668..670171	-	504	WP_001333820.1	M48 family metallopeptidase	-
GTK51_RS03320 (670256)	670256..671392	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
GTK51_RS03325 (671676)	671676..671990	+	315	WP_063501682.1	type II toxin-antitoxin system toxin HigB	Toxin
GTK51_RS03330 (671987)	671987..672403	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
GTK51_RS03335 (672448)	672448..674466	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
GTK51_RS03340 (674892)	674892..677243	-	2352	WP_000695487.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12202.24 Da
• Isoelectric Point: 10.1604

>T146273 WP_063501682.1 NZ_CP047469:671676-671990 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALRNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T146273 NZ_CP062736:365365-365640 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT146273 WP_000560266.1 NZ_CP047469:671987-672403 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT146273 NZ_CP062736:365637-365996 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A5P0J3S6