Detailed information of TA system TA146270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3962716..3963410
• Replicon: chromosome
• Accession: NZ_CP047461
• Organism: Escherichia coli strain ZF34

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: GTK64_RS19010
• Protein ID: WP_001263493.1
• Coordinates: 3963012..3963410 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: GTK64_RS19005
• Protein ID: WP_000554757.1
• Coordinates: 3962716..3963009 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK64_RS18980	3958533..3959000	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
GTK64_RS18985	3959020..3959736	+	717	WP_130065490.1	FliA/WhiG family RNA polymerase sigma factor	-
GTK64_RS18990	3959749..3960612	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
GTK64_RS18995	3960615..3961538	+	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
GTK64_RS19000	3961609..3962664	+	1056	WP_001226164.1	DNA polymerase IV	-
GTK64_RS19005	3962716..3963009	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
GTK64_RS19010	3963012..3963410	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
GTK64_RS19015	3963420..3963872	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
GTK64_RS19020	3964118..3964321	+	204	Protein_3692	RNA ligase RtcB family protein	-
GTK64_RS19025	3964320..3964841	+	522	Protein_3693	peptide chain release factor H	-
GTK64_RS19030	3964898..3966355	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
GTK64_RS19035	3966616..3967074	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
GTK64_RS19040	3967166..3968410	+	1245	WP_000189541.1	esterase FrsA	-
-	3967670..3967750	+	81	NuclAT_11	-	-
-	3967670..3967750	+	81	NuclAT_11	-	-
-	3967670..3967750	+	81	NuclAT_11	-	-
-	3967670..3967750	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T146270 WP_001263493.1 NZ_CP047461:3963012-3963410 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T146270 NZ_CP062734:74038-74196 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT146270 WP_000554757.1 NZ_CP047461:3962716-3963009 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT146270 NZ_CP062734:73775-73973 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6