Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 2245305..2245998 Replicon chromosome
Accession NZ_CP047461
Organism Escherichia coli strain ZF34

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag GTK64_RS10775 Protein ID WP_000415584.1
Coordinates 2245702..2245998 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag GTK64_RS10770 Protein ID WP_000650107.1
Coordinates 2245305..2245700 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK64_RS10760 2241200..2243458 - 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
GTK64_RS10765 2243596..2245172 - 1577 Protein_2098 ABC transporter substrate-binding protein -
GTK64_RS10770 2245305..2245700 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
GTK64_RS10775 2245702..2245998 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
GTK64_RS10780 2246203..2246685 - 483 WP_000183505.1 transcriptional regulator -
GTK64_RS10785 2246738..2247130 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
GTK64_RS10790 2247282..2247941 + 660 WP_001221493.1 two-component system response regulator QseB -
GTK64_RS10795 2247938..2249287 + 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
GTK64_RS10800 2249333..2249665 - 333 WP_000917684.1 DUF2645 family protein -
GTK64_RS10805 2249984..2250565 + 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
GTK64_RS10810 2250596..2250910 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - rfaE 2125533..2269994 144461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T146262 WP_000415584.1 NZ_CP047461:c2245998-2245702 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T146262 NZ_CP062733:c4469042-4468644 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT146262 WP_000650107.1 NZ_CP047461:c2245700-2245305 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT146262 NZ_CP062733:c4469338-4469045 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References