Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mqsRA (relBE)/MqsR-MqsA
Location 2244628..2245321 Replicon chromosome
Accession NZ_CP047455
Organism Escherichia coli strain ZF31

Toxin (Protein)


Gene name mqsR Uniprot ID S1EZG2
Locus tag GTK48_RS10770 Protein ID WP_000415584.1
Coordinates 2245025..2245321 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name mqsA Uniprot ID S1EBV2
Locus tag GTK48_RS10765 Protein ID WP_000650107.1
Coordinates 2244628..2245023 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTK48_RS10755 2240523..2242781 - 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
GTK48_RS10760 2242919..2244495 - 1577 Protein_2099 ABC transporter substrate-binding protein -
GTK48_RS10765 2244628..2245023 - 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA Antitoxin
GTK48_RS10770 2245025..2245321 - 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR Toxin
GTK48_RS10775 2245526..2246008 - 483 WP_000183505.1 transcriptional regulator -
GTK48_RS10780 2246061..2246453 - 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
GTK48_RS10785 2246605..2247264 + 660 WP_001221493.1 two-component system response regulator QseB -
GTK48_RS10790 2247261..2248610 + 1350 WP_000673402.1 two-component system sensor histidine kinase QseC -
GTK48_RS10795 2248656..2248988 - 333 WP_000917684.1 DUF2645 family protein -
GTK48_RS10800 2249307..2249888 + 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
GTK48_RS10805 2249919..2250233 + 315 WP_000958598.1 antibiotic biosynthesis monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - rfaE 2124856..2269317 144461


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin MqsR

(5-97)

Antitoxin

Zinc finger, MqsA-type

(3-47)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11231.96 Da        Isoelectric Point: 8.9070

>T146239 WP_000415584.1 NZ_CP047455:c2245321-2245025 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Download         Length: 297 bp

>T146239 NZ_CP062732:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14703.10 Da        Isoelectric Point: 9.2136

>AT146239 WP_000650107.1 NZ_CP047455:c2245023-2244628 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Download         Length: 396 bp

>AT146239 NZ_CP062732:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3HI2

Antitoxin

Source ID Structure
PDB 3HI2
PDB 3GA8
PDB 3O9X
PDB 2KZ8
PDB 3FMY
PDB 3GN5
AlphaFold DB A0A7U9LU65

References