Detailed information of TA system TA146225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4401375..4402129
• Replicon: chromosome
• Accession: NZ_CP047424
• Organism: Salmonella enterica subsp. enterica serovar Dessau strain KUFSE-SAL0043

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: GTK42_RS21210
• Protein ID: WP_000558166.1
• Coordinates: 4401375..4401686 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTK42_RS21215
• Protein ID: WP_001259011.1
• Coordinates: 4401683..4402129 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK42_RS21180 (4397033)	4397033..4397935	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
GTK42_RS21185 (4397932)	4397932..4398567	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
GTK42_RS21190 (4398564)	4398564..4399493	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
GTK42_RS21195 (4399540)	4399540..4399830	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
GTK42_RS21200 (4399831)	4399831..4400142	-	312	WP_001159628.1	hypothetical protein	-
GTK42_RS21205 (4400360)	4400360..4401289	+	930	WP_001127701.1	alpha/beta hydrolase	-
GTK42_RS21210 (4401375)	4401375..4401686	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
GTK42_RS21215 (4401683)	4401683..4402129	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
GTK42_RS21220 (4402144)	4402144..4403085	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
GTK42_RS21225 (4403130)	4403130..4403567	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
GTK42_RS21230 (4403564)	4403564..4404436	-	873	WP_000921423.1	virulence factor BrkB family protein	-
GTK42_RS21235 (4404430)	4404430..4405029	-	600	WP_000965695.1	glucose-1-phosphatase	-
GTK42_RS21240 (4405220)	4405220..4406023	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
GTK42_RS21245 (4406057)	4406057..4406953	-	897	WP_001805206.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T146225 WP_000558166.1 NZ_CP047424:4401375-4401686 [Salmonella enterica subsp. enterica serovar Dessau]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T146225 NZ_CP062731:3062424-3062702 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT146225 WP_001259011.1 NZ_CP047424:4401683-4402129 [Salmonella enterica subsp. enterica serovar Dessau]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT146225 NZ_CP062731:3062231-3062422 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90