Detailed information of TA system TA146224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4399540..4400142
• Replicon: chromosome
• Accession: NZ_CP047424
• Organism: Salmonella enterica subsp. enterica serovar Dessau strain KUFSE-SAL0043

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3V4SQH3
• Locus tag: GTK42_RS21200
• Protein ID: WP_001159628.1
• Coordinates: 4399831..4400142 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTK42_RS21195
• Protein ID: WP_000362050.1
• Coordinates: 4399540..4399830 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTK42_RS21180 (4397033)	4397033..4397935	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
GTK42_RS21185 (4397932)	4397932..4398567	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
GTK42_RS21190 (4398564)	4398564..4399493	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
GTK42_RS21195 (4399540)	4399540..4399830	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
GTK42_RS21200 (4399831)	4399831..4400142	-	312	WP_001159628.1	hypothetical protein	Toxin
GTK42_RS21205 (4400360)	4400360..4401289	+	930	WP_001127701.1	alpha/beta hydrolase	-
GTK42_RS21210 (4401375)	4401375..4401686	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
GTK42_RS21215 (4401683)	4401683..4402129	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
GTK42_RS21220 (4402144)	4402144..4403085	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
GTK42_RS21225 (4403130)	4403130..4403567	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
GTK42_RS21230 (4403564)	4403564..4404436	-	873	WP_000921423.1	virulence factor BrkB family protein	-
GTK42_RS21235 (4404430)	4404430..4405029	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12376.29 Da
• Isoelectric Point: 9.4460

>T146224 WP_001159628.1 NZ_CP047424:c4400142-4399831 [Salmonella enterica subsp. enterica serovar Dessau]
MQFIETELFTEDVKKLLDDDEYHKFQVFMAQHPDCGDVIQETGGLRKMRWGSRGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T146224 NZ_CP062731:c2852707-2852531 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT146224 WP_000362050.1 NZ_CP047424:c4399830-4399540 [Salmonella enterica subsp. enterica serovar Dessau]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT146224 NZ_CP062731:c2852485-2852069 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A3V4SQH3