Detailed information of TA system TA146199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 117454..118111
• Replicon: plasmid p18064-65-CSB-B-B
• Accession: NZ_CP047421
• Organism: Shewanella algae strain 18064-CSB-B-B

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: GM320_RS00635
• Protein ID: WP_000270043.1
• Coordinates: 117761..118111 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GM320_RS00630
• Protein ID: WP_000124640.1
• Coordinates: 117454..117756 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GM320_RS00580	113088..113516	+	429	WP_000591076.1	hypothetical protein	-
GM320_RS00585	113574..113933	+	360	WP_000422769.1	hypothetical protein	-
GM320_RS00590	113933..114379	+	447	WP_000919343.1	hypothetical protein	-
GM320_RS00595	114376..114894	+	519	WP_000210757.1	nitrite reductase	-
GM320_RS00600	114894..115124	+	231	WP_000972665.1	hypothetical protein	-
GM320_RS00605	115111..115968	+	858	WP_001167036.1	hypothetical protein	-
GM320_RS22180	115880..116185	+	306	WP_171820680.1	hypothetical protein	-
GM320_RS00615	116188..116715	+	528	WP_001210659.1	thermonuclease family protein	-
GM320_RS00620	116773..117045	+	273	WP_001043046.1	HU family DNA-binding protein	-
GM320_RS00625	117134..117427	+	294	WP_001239998.1	hypothetical protein	-
GM320_RS00630	117454..117756	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
GM320_RS00635	117761..118111	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GM320_RS00640	118274..118822	+	549	WP_001061573.1	hypothetical protein	-
GM320_RS00645	119163..119357	+	195	WP_000343597.1	hypothetical protein	-
GM320_RS22185	119368..119739	+	372	Protein_133	hypothetical protein	-
GM320_RS00650	119732..120202	+	471	WP_001281821.1	hypothetical protein	-
GM320_RS00655	120217..120552	-	336	WP_000683477.1	hypothetical protein	-
GM320_RS00660	120649..121137	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
GM320_RS00665	121140..121637	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / qacE / sul1 / armA / blaCTX-M-15	-	1..149553	149553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T146199 WP_000270043.1 NZ_CP047421:c118111-117761 [Shewanella algae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T146199 NZ_CP062729:c3366009-3365635 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT146199 WP_000124640.1 NZ_CP047421:c117756-117454 [Shewanella algae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT146199 NZ_CP062729:c3366430-3366056 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9