Detailed information of TA system TA146196

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22425..22694
• Replicon: plasmid pMS6193B
• Accession: NZ_CP047407
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: MS6193_RS24800
• Protein ID: WP_001312861.1
• Coordinates: 22536..22694 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22425..22490 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS24760	17770..17958	+	189	WP_032146593.1	hypothetical protein	-
MS6193_RS24765	17960..18166	+	207	WP_000275858.1	hypothetical protein	-
MS6193_RS24770	18192..18731	+	540	WP_001825195.1	single-stranded DNA-binding protein	-
MS6193_RS24775	18794..19027	+	234	WP_000005990.1	DUF905 family protein	-
MS6193_RS24780	19093..21051	+	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
MS6193_RS24785	21106..21540	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
MS6193_RS24790	21537..22256	+	720	WP_013362833.1	plasmid SOS inhibition protein A	-
MS6193_RS24795	22268..22456	-	189	WP_001299721.1	hypothetical protein	-
-	22268..22492	+	225	NuclAT_0	-	-
-	22268..22492	+	225	NuclAT_0	-	-
-	22268..22492	+	225	NuclAT_0	-	-
-	22268..22492	+	225	NuclAT_0	-	-
-	22425..22490	+	66	-	-	Antitoxin
MS6193_RS24800	22536..22694	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
MS6193_RS24805	23195..23383	+	189	WP_042111937.1	hypothetical protein	-
MS6193_RS24810	23616..23903	+	288	WP_000107544.1	hypothetical protein	-
MS6193_RS24815	24021..24842	+	822	WP_001234469.1	DUF945 domain-containing protein	-
MS6193_RS24820	25139..25786	-	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
MS6193_RS24825	26063..26446	+	384	WP_000124981.1	relaxosome protein TraM	-
MS6193_RS24830	26637..27323	+	687	WP_000332484.1	PAS domain-containing protein	-
MS6193_RS24835	27417..27644	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB	-	1..76661	76661

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T146196 WP_001312861.1 NZ_CP047407:22536-22694 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T146196 NZ_CP062729:3061110-3061388 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT146196 NZ_CP047407:22425-22490 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin