Detailed information of TA system TA146188

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29757..30178
• Replicon: plasmid pMS6193A-NDM
• Accession: NZ_CP047406
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: MS6193_RS23950
• Protein ID: WP_001302184.1
• Coordinates: 30020..30178 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	29757..29955 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS23915	25453..26847	+	1395	WP_001037799.1	carbohydrate porin	-
MS6193_RS23920	27093..27197	-	105	Protein_28	thiosulfate reductase cytochrome B subunit	-
MS6193_RS23925	27254..27947	+	694	Protein_29	IS1 family transposase	-
MS6193_RS23930	27963..28586	+	624	WP_000912556.1	potassium channel family protein	-
MS6193_RS23935	28671..28922	+	252	Protein_31	iron-containing alcohol dehydrogenase	-
MS6193_RS23940	29211..29528	-	318	Protein_32	IS110 family transposase	-
MS6193_RS23945	29683..29788	+	106	Protein_33	plasmid SOS inhibition protein A	-
-	29757..29955	+	199	NuclAT_0	-	Antitoxin
-	29757..29955	+	199	NuclAT_0	-	Antitoxin
-	29757..29955	+	199	NuclAT_0	-	Antitoxin
-	29757..29955	+	199	NuclAT_0	-	Antitoxin
MS6193_RS23950	30020..30178	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
MS6193_RS23955	30846..31052	+	207	WP_000275854.1	hypothetical protein	-
MS6193_RS23960	31077..31364	+	288	WP_000107242.1	hypothetical protein	-
MS6193_RS23965	31483..32304	+	822	WP_001234465.1	DUF945 domain-containing protein	-
MS6193_RS23970	32600..33172	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
MS6193_RS23975	33542..33925	+	384	WP_001063020.1	relaxosome protein TraM	-
MS6193_RS23980	34116..34763	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
MS6193_RS23985	34899..35114	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaNDM-1 / sul1 / qacE / aadA2 / dfrA12	-	1..142890	142890
	-	flank	IS/Tn	-	-	27570..27947	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T146188 WP_001302184.1 NZ_CP047406:30020-30178 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T146188 NZ_CP062729:c457211-456726 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT146188 NZ_CP047406:29757-29955 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin