Detailed information of TA system TA146183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4306882..4307484
• Replicon: chromosome
• Accession: NZ_CP047405
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MS6193_RS20865
• Protein ID: WP_000897305.1
• Coordinates: 4306882..4307193 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MS6193_RS20870
• Protein ID: WP_000356397.1
• Coordinates: 4307194..4307484 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS20840	4302796..4303395	+	600	WP_001295269.1	glucose-1-phosphatase	-
MS6193_RS20845	4303389..4304261	+	873	WP_000920762.1	virulence factor BrkB family protein	-
MS6193_RS20850	4304258..4304695	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
MS6193_RS20855	4304740..4305681	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MS6193_RS20860	4305745..4306653	-	909	WP_001162704.1	alpha/beta hydrolase	-
MS6193_RS20865	4306882..4307193	+	312	WP_000897305.1	hypothetical protein	Toxin
MS6193_RS20870	4307194..4307484	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
MS6193_RS20875	4308070..4308288	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
MS6193_RS20880	4308507..4308749	+	243	WP_001087409.1	hypothetical protein	-
MS6193_RS20885	4309079..4310008	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
MS6193_RS20890	4310005..4310640	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MS6193_RS20895	4310637..4311539	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T146183 WP_000897305.1 NZ_CP047405:4306882-4307193 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T146183 NZ_CP062728:71583-71741 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT146183 WP_000356397.1 NZ_CP047405:4307194-4307484 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT146183 NZ_CP062728:71320-71518 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7