Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3496863..3497662 Replicon chromosome
Accession NZ_CP047405
Organism Escherichia coli strain MS6193

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag MS6193_RS17000 Protein ID WP_000347266.1
Coordinates 3497198..3497662 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MS6193_RS16995 Protein ID WP_001307405.1
Coordinates 3496863..3497198 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6193_RS16980 3492648..3493418 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
MS6193_RS16985 3493434..3494768 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MS6193_RS16990 3495143..3496714 + 1572 WP_001273741.1 galactarate dehydratase -
MS6193_RS16995 3496863..3497198 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MS6193_RS17000 3497198..3497662 + 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MS6193_RS17005 3497717..3498526 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MS6193_RS17010 3498775..3500055 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
MS6193_RS17015 3500078..3500551 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MS6193_RS17020 3500562..3501341 + 780 WP_174190366.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
MS6193_RS17025 3501331..3502209 + 879 WP_001315856.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
MS6193_RS17030 3502227..3502661 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3486207..3497662 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T146181 WP_000347266.1 NZ_CP047405:3497198-3497662 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T146181 NZ_CP062728:2066-2341 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT146181 WP_001307405.1 NZ_CP047405:3496863-3497198 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT146181 NZ_CP062728:1782-2066 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References