Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2275216..2275718 Replicon chromosome
Accession NZ_CP047405
Organism Escherichia coli strain MS6193

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MS6193_RS11170 Protein ID WP_000767829.1
Coordinates 2275216..2275470 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MS6193_RS11175 Protein ID WP_001259255.1
Coordinates 2275467..2275718 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6193_RS11140 2270231..2270458 - 228 WP_000985278.1 sulfurtransferase TusA family protein -
MS6193_RS11145 2270472..2271530 - 1059 WP_000492312.1 transport protein YeeE -
MS6193_RS11150 2271709..2273067 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MS6193_RS11155 2273057..2273119 - 63 WP_010723108.1 membrane protein YoeI -
MS6193_RS11160 2273334..2274263 - 930 WP_000803351.1 LysR family transcriptional regulator -
MS6193_RS11165 2274309..2275133 - 825 WP_000754737.1 SDR family oxidoreductase -
MS6193_RS11170 2275216..2275470 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MS6193_RS11175 2275467..2275718 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MS6193_RS11180 2276001..2276051 + 51 WP_001364200.1 his operon leader peptide -
MS6193_RS11185 2276197..2277096 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
MS6193_RS11190 2277102..2278406 + 1305 WP_074499979.1 histidinol dehydrogenase -
MS6193_RS11195 2278403..2279473 + 1071 WP_000108986.1 histidinol-phosphate transaminase -
MS6193_RS11200 2279473..2280540 + 1068 WP_016232166.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T146177 WP_000767829.1 NZ_CP047405:c2275470-2275216 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T146177 NZ_CP062727:c4706366-4706052 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGTAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT146177 WP_001259255.1 NZ_CP047405:c2275718-2275467 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT146177 NZ_CP062727:c4706602-4706369 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References