Detailed information of TA system TA146162

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1519492..1519863
• Replicon: chromosome
• Accession: NZ_CP047405
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: MS6193_RS07330
• Protein ID: WP_001317028.1
• Coordinates: 1519669..1519863 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1519492..1519670 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS07305	1515447..1516820	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
MS6193_RS07310	1516949..1517884	-	936	WP_001153728.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
MS6193_RS07315	1517936..1519171	-	1236	WP_000040852.1	site-specific integrase	-
MS6193_RS07320	1519173..1519388	-	216	WP_000079604.1	excisionase XisR	-
MS6193_RS07325	1519467..1519676	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1519492..1519670	+	179	NuclAT_0	-	Antitoxin
-	1519492..1519670	+	179	NuclAT_0	-	Antitoxin
-	1519492..1519670	+	179	NuclAT_0	-	Antitoxin
-	1519492..1519670	+	179	NuclAT_0	-	Antitoxin
MS6193_RS07330	1519669..1519863	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
MS6193_RS07335	1519947..1520729	-	783	WP_060615110.1	recombination protein RecT	-
MS6193_RS07340	1520722..1523322	-	2601	WP_000105127.1	exodeoxyribonuclease VIII	-
MS6193_RS07345	1523424..1523699	-	276	WP_000632297.1	protein RacC	-
MS6193_RS07350	1523774..1523944	-	171	WP_001352098.1	conserved protein, Rac prophage	-
MS6193_RS07355	1523944..1524165	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1517936..1528459	10523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T146162 WP_001317028.1 NZ_CP047405:c1519863-1519669 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T146162 NZ_CP062727:946470-946877 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT146162 NZ_CP047405:1519492-1519670 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin