Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 3310404..3311061 Replicon chromosome
Accession NZ_CP047391
Organism Serratia marcescens strain 1602

Toxin (Protein)


Gene name higB Uniprot ID A0A0M4RY59
Locus tag GRX59_RS15785 Protein ID WP_060420873.1
Coordinates 3310738..3311061 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0M5M3W3
Locus tag GRX59_RS15780 Protein ID WP_060420390.1
Coordinates 3310404..3310700 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRX59_RS15755 3305928..3306563 + 636 WP_060437753.1 flavodoxin family protein -
GRX59_RS15760 3306707..3307816 + 1110 WP_060419067.1 glycosyltransferase family 9 protein -
GRX59_RS15765 3307746..3308963 - 1218 WP_060419069.1 O-antigen ligase family protein -
GRX59_RS15770 3308941..3309849 - 909 WP_060419071.1 sugar glycosyltransferase -
GRX59_RS15775 3310154..3310396 + 243 WP_025303447.1 hypothetical protein -
GRX59_RS15780 3310404..3310700 - 297 WP_060420390.1 helix-turn-helix domain-containing protein Antitoxin
GRX59_RS15785 3310738..3311061 - 324 WP_060420873.1 toxin HigB-2 Toxin
GRX59_RS15790 3311126..3311755 - 630 WP_060425958.1 hypothetical protein -
GRX59_RS15795 3311841..3312776 + 936 WP_049212009.1 LysR family transcriptional regulator -
GRX59_RS15800 3312759..3313406 - 648 WP_019452474.1 DNA oxidative demethylase AlkB -
GRX59_RS15805 3313546..3314424 + 879 WP_049273981.1 EamA family transporter RarD -
GRX59_RS15810 3314426..3315184 - 759 WP_039565253.1 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-83)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12370.15 Da        Isoelectric Point: 8.8132

>T146145 WP_060420873.1 NZ_CP047391:c3311061-3310738 [Serratia marcescens]
MDYLEFIETTVFSRKRAELMKDDDFQAFQSYLLLNHHEGDTISHTGGCKKIRWGLDGKGKRGGVRVIYYLTAPSGRVYLL
LIYRKNEKDDLSGQEKAILRSIVQQLD

Download         Length: 324 bp

>T146145 NZ_CP062725:4289149-4289367 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11003.00 Da        Isoelectric Point: 10.1906

>AT146145 WP_060420390.1 NZ_CP047391:c3310700-3310404 [Serratia marcescens]
MKKELFDDLVSSMNEMVAIEKGKMTPKSEHVHRHAIPDVKLLRKSRGMKQAEFAKAVGASVGLVQSWEQERRIPSGSALK
LLLLIERNPALLDELLVI

Download         Length: 297 bp

>AT146145 NZ_CP062725:4288749-4289123 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4RY59

Antitoxin

Source ID Structure
AlphaFold DB A0A0M5M3W3

References