Detailed information of TA system TA146118

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36780..37050
• Replicon: plasmid pCAU16175_3
• Accession: NZ_CP047381
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GTP13_RS30710
• Protein ID: WP_001312861.1
• Coordinates: 36892..37050 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36780..36843 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS30675	32081..32295	-	215	Protein_41	hypothetical protein	-
GTP13_RS31225	32323..32529	+	207	WP_112899861.1	single-stranded DNA-binding protein	-
GTP13_RS30680	32555..33082	+	528	WP_000290816.1	single-stranded DNA-binding protein	-
GTP13_RS30685	33138..33371	+	234	WP_000005971.1	DUF905 domain-containing protein	-
GTP13_RS30690	33435..35393	+	1959	Protein_45	ParB/RepB/Spo0J family partition protein	-
GTP13_RS30695	35462..35896	+	435	WP_001623019.1	conjugation system SOS inhibitor PsiB	-
GTP13_RS30700	35893..36612	+	720	WP_001629131.1	plasmid SOS inhibition protein A	-
GTP13_RS30705	36624..36812	-	189	WP_001299721.1	hypothetical protein	-
-	36624..36848	+	225	NuclAT_0	-	-
-	36624..36848	+	225	NuclAT_0	-	-
-	36624..36848	+	225	NuclAT_0	-	-
-	36624..36848	+	225	NuclAT_0	-	-
-	36780..36843	-	64	-	-	Antitoxin
GTP13_RS30710	36892..37050	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
GTP13_RS31230	37741..37947	+	207	WP_000547971.1	hypothetical protein	-
GTP13_RS30720	37972..38259	+	288	WP_000107535.1	hypothetical protein	-
GTP13_RS30725	38377..39198	+	822	WP_137509722.1	DUF945 domain-containing protein	-
GTP13_RS30730	39495..40142	-	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
GTP13_RS30735	40419..40802	+	384	WP_000124981.1	relaxosome protein TraM	-
GTP13_RS30740	40993..41679	+	687	WP_073520017.1	PAS domain-containing protein	-
GTP13_RS30745	41773..42000	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-1.1 / blaTEM-1B / tet(M)	-	1..76633	76633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T146118 WP_001312861.1 NZ_CP047381:36892-37050 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T146118 NZ_CP062723:c4474572-4474174 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 64 bp

>AT146118 NZ_CP047381:c36843-36780 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin