Detailed information of TA system TA146117

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31273..31619
• Replicon: plasmid pCAU16175_3
• Accession: NZ_CP047381
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: GTP13_RS30670
• Protein ID: WP_012817833.1
• Coordinates: 31461..31619 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	31273..31409 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS30640	26702..27235	+	534	WP_053913426.1	single-stranded DNA-binding protein	-
GTP13_RS30645	27293..27526	+	234	WP_053913425.1	DUF905 family protein	-
GTP13_RS30650	27590..29548	+	1959	WP_167583057.1	ParB/RepB/Spo0J family partition protein	-
GTP13_RS30655	29617..30051	+	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
GTP13_RS30660	30048..30767	+	720	WP_053913421.1	plasmid SOS inhibition protein A	-
GTP13_RS30665	30767..31285	+	519	WP_112899860.1	hypothetical protein	-
-	31261..31409	+	149	NuclAT_1	-	-
-	31261..31409	+	149	NuclAT_1	-	-
-	31261..31409	+	149	NuclAT_1	-	-
-	31261..31409	+	149	NuclAT_1	-	-
-	31273..31409	-	137	-	-	Antitoxin
GTP13_RS30670	31461..31619	+	159	WP_012817833.1	type I toxin-antitoxin system Hok family toxin	Toxin
GTP13_RS30675	32081..32295	-	215	Protein_41	hypothetical protein	-
GTP13_RS31225	32323..32529	+	207	WP_112899861.1	single-stranded DNA-binding protein	-
GTP13_RS30680	32555..33082	+	528	WP_000290816.1	single-stranded DNA-binding protein	-
GTP13_RS30685	33138..33371	+	234	WP_000005971.1	DUF905 domain-containing protein	-
GTP13_RS30690	33435..35393	+	1959	Protein_45	ParB/RepB/Spo0J family partition protein	-
GTP13_RS30695	35462..35896	+	435	WP_001623019.1	conjugation system SOS inhibitor PsiB	-
GTP13_RS30700	35893..36612	+	720	WP_001629131.1	plasmid SOS inhibition protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-1.1 / blaTEM-1B / tet(M)	-	1..76633	76633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6078.30 Da
• Isoelectric Point: 8.7390

>T146117 WP_012817833.1 NZ_CP047381:31461-31619 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLMFTYLTRKSLCEIRYRDGDREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T146117 NZ_CP062723:4215909-4216127 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 137 bp

>AT146117 NZ_CP047381:c31409-31273 [Escherichia coli]
AGACATATGGGATGCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTATCTTCTTTCTGCCACACAACACGGTAACAAA
CCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATAT

Structures

Antitoxin