Detailed information of TA system TA146110

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5410427..5411029
• Replicon: chromosome
• Accession: NZ_CP047378
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GTP13_RS27535
• Protein ID: WP_000897305.1
• Coordinates: 5410718..5411029 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTP13_RS27530
• Protein ID: WP_000356397.1
• Coordinates: 5410427..5410717 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS27505	5405973..5406608	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
GTP13_RS27510	5406605..5407534	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GTP13_RS27515	5407934..5408914	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
GTP13_RS27520	5409143..5409385	-	243	WP_001086388.1	hypothetical protein	-
GTP13_RS27525	5409604..5409822	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
GTP13_RS27530	5410427..5410717	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GTP13_RS27535	5410718..5411029	-	312	WP_000897305.1	hypothetical protein	Toxin
GTP13_RS27540	5411258..5412166	+	909	WP_001162704.1	alpha/beta hydrolase	-
GTP13_RS27545	5412230..5413171	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GTP13_RS27550	5413216..5413653	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GTP13_RS27555	5413650..5414522	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GTP13_RS27560	5414516..5415115	-	600	WP_001315111.1	glucose-1-phosphatase	-
GTP13_RS27565	5415214..5415999	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5407934..5408914	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T146110 WP_000897305.1 NZ_CP047378:c5411029-5410718 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T146110 NZ_CP062723:3066077-3066355 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT146110 WP_000356397.1 NZ_CP047378:c5410717-5410427 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT146110 NZ_CP062723:3065884-3066075 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7