Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4917656..4918455 Replicon chromosome
Accession NZ_CP047378
Organism Escherichia coli strain CAU16175

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag GTP13_RS25145 Protein ID WP_000347273.1
Coordinates 4917991..4918455 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag GTP13_RS25140 Protein ID WP_001307405.1
Coordinates 4917656..4917991 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTP13_RS25125 4913441..4914211 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
GTP13_RS25130 4914227..4915561 - 1335 WP_096245719.1 galactarate/glucarate/glycerate transporter GarP -
GTP13_RS25135 4915936..4917507 + 1572 WP_096245720.1 galactarate dehydratase -
GTP13_RS25140 4917656..4917991 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
GTP13_RS25145 4917991..4918455 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
GTP13_RS25150 4918510..4919319 - 810 WP_000072193.1 aga operon transcriptional regulator AgaR -
GTP13_RS25155 4919568..4920848 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
GTP13_RS25160 4920871..4921344 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
GTP13_RS25165 4921355..4922134 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
GTP13_RS25170 4922124..4922321 + 198 Protein_4907 PTS system mannose/fructose/sorbose family transporter subunit IID -
GTP13_RS25175 4922379..4923073 + 695 WP_085949012.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4907000..4925468 18468


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T146109 WP_000347273.1 NZ_CP047378:4917991-4918455 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T146109 NZ_CP062723:c2855051-2854875 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT146109 WP_001307405.1 NZ_CP047378:4917656-4917991 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT146109 NZ_CP062723:c2854829-2854413 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References