Detailed information of TA system TA146105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4385956..4386683
• Replicon: chromosome
• Accession: NZ_CP047378
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: GTP13_RS22605
• Protein ID: WP_000547559.1
• Coordinates: 4386372..4386683 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTP13_RS22600
• Protein ID: WP_000126294.1
• Coordinates: 4385956..4386375 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS22580	4381006..4381533	-	528	WP_001078781.1	electron transport protein HydN	-
GTP13_RS22585	4381682..4382692	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
GTP13_RS22590	4382952..4384409	+	1458	WP_096245361.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
GTP13_RS22595	4384418..4385842	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
GTP13_RS22600	4385956..4386375	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
GTP13_RS22605	4386372..4386683	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
GTP13_RS22610	4386857..4387600	-	744	WP_001341829.1	hypothetical protein	-
GTP13_RS22615	4387643..4388113	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
GTP13_RS22620	4388106..4388516	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
GTP13_RS22625	4388513..4389280	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
GTP13_RS22630	4389280..4389822	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
GTP13_RS22635	4389832..4391541	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T146105 WP_000547559.1 NZ_CP047378:c4386683-4386372 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T146105 NZ_CP062723:946459-946866 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT146105 WP_000126294.1 NZ_CP047378:c4386375-4385956 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT146105 NZ_CP062723:946212-946478 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163