Detailed information of TA system TA146089

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2397142..2397513
• Replicon: chromosome
• Accession: NZ_CP047378
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: Q8Z5Y6
• Locus tag: GTP13_RS12035
• Protein ID: WP_010989194.1
• Coordinates: 2397319..2397513 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2397142..2397320 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS11995	2392584..2393240	-	657	WP_000011658.1	carbon-nitrogen hydrolase family protein	-
GTP13_RS12000	2393342..2393572	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
GTP13_RS12005	2393711..2394085	+	375	WP_096245560.1	CopC domain-containing protein YobA	-
GTP13_RS12010	2394089..2394961	+	873	WP_000879280.1	copper homeostasis membrane protein CopD	-
GTP13_RS12015	2394974..2395315	+	342	WP_000976492.1	YebY family protein	-
GTP13_RS12020	2395708..2396784	-	1077	WP_001189085.1	phage integrase Arm DNA-binding domain-containing protein	-
GTP13_RS12025	2396750..2397031	-	282	WP_097412098.1	excisionase	-
GTP13_RS12030	2397138..2397326	-	189	WP_024177035.1	DUF1187 family protein	-
-	2397142..2397320	+	179	NuclAT_1	-	Antitoxin
-	2397142..2397320	+	179	NuclAT_1	-	Antitoxin
-	2397142..2397320	+	179	NuclAT_1	-	Antitoxin
-	2397142..2397320	+	179	NuclAT_1	-	Antitoxin
GTP13_RS12035	2397319..2397513	-	195	WP_010989194.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
GTP13_RS12040	2397577..2398629	-	1053	WP_001004413.1	recombinase RecT	-
GTP13_RS12045	2398641..2401790	-	3150	WP_167582685.1	PD-(D/E)XK nuclease-like domain-containing protein	-
GTP13_RS12050	2401891..2402166	-	276	WP_044805262.1	hypothetical protein	-
GTP13_RS12055	2402241..2402411	-	171	WP_000358365.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	2382366..2464499	82133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7077.01 Da
• Isoelectric Point: 9.2886

>T146089 WP_010989194.1 NZ_CP047378:c2397513-2397319 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T146089 NZ_CP062721:c4514855-4514457 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT146089 NZ_CP047378:2397142-2397320 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCTTGCTTCTTTTTTCCCAGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCAGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A716R3V7

Antitoxin