Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1108736..1109271 Replicon chromosome
Accession NZ_CP047378
Organism Escherichia coli strain CAU16175

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag GTP13_RS05355 Protein ID WP_032183258.1
Coordinates 1108736..1109023 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag GTP13_RS05360 Protein ID WP_000729704.1
Coordinates 1109011..1109271 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTP13_RS05340 1106318..1106896 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
GTP13_RS05345 1107102..1107869 + 768 WP_000333380.1 class II glutamine amidotransferase -
GTP13_RS05350 1107840..1108580 - 741 WP_001225679.1 murein L,D-transpeptidase -
GTP13_RS05355 1108736..1109023 - 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
GTP13_RS05360 1109011..1109271 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
GTP13_RS05365 1109457..1110230 + 774 WP_000543897.1 C40 family peptidase -
GTP13_RS05370 1110287..1110643 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
GTP13_RS05375 1110636..1110914 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
GTP13_RS05380 1111019..1113113 - 2095 Protein_1043 flagellar biosynthesis protein FlhA -
GTP13_RS05385 1113097..1114236 - 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T146077 WP_032183258.1 NZ_CP047378:c1109023-1108736 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T146077 NZ_CP062721:1093239-1093574 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT146077 WP_000729704.1 NZ_CP047378:c1109271-1109011 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT146077 NZ_CP062721:1092991-1093239 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References