Detailed information of TA system TA146075

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 749786..750198
• Replicon: chromosome
• Accession: NZ_CP047378
• Organism: Escherichia coli strain CAU16175

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0F3WCK2
• Locus tag: GTP13_RS03725
• Protein ID: WP_000132621.1
• Coordinates: 749786..750127 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	750122..750198 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTP13_RS03710	745796..747208	-	1413	WP_000199352.1	PLP-dependent aminotransferase family protein	-
GTP13_RS03715	747385..747549	+	165	WP_000394276.1	DUF1127 domain-containing protein	-
GTP13_RS03720	747646..749739	+	2094	WP_000648228.1	DUF262 domain-containing protein	-
GTP13_RS03725	749786..750127	-	342	WP_000132621.1	endoribonuclease SymE	Toxin
-	750122..750198	+	77	NuclAT_27	-	Antitoxin
-	750122..750198	+	77	NuclAT_27	-	Antitoxin
-	750122..750198	+	77	NuclAT_27	-	Antitoxin
-	750122..750198	+	77	NuclAT_27	-	Antitoxin
-	750122..750198	+	77	NuclAT_28	-	Antitoxin
-	750122..750198	+	77	NuclAT_28	-	Antitoxin
-	750122..750198	+	77	NuclAT_28	-	Antitoxin
-	750122..750198	+	77	NuclAT_28	-	Antitoxin
GTP13_RS03730	750348..752117	-	1770	WP_000110079.1	restriction endonuclease subunit S	-
GTP13_RS03735	752117..753586	-	1470	WP_001300455.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12342.12 Da
• Isoelectric Point: 7.8218

>T146075 WP_000132621.1 NZ_CP047378:c750127-749786 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T146075 NZ_CP062721:751331-751645 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT146075 NZ_CP047378:750122-750198 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0F3WCK2

Antitoxin