Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4104037..4104634 Replicon chromosome
Accession NZ_CP047360
Organism Klebsiella variicola strain AHKv-S01

Toxin (Protein)


Gene name higB Uniprot ID A0A0B7GEF2
Locus tag GSY73_RS20250 Protein ID WP_012542526.1
Coordinates 4104317..4104634 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6H0BIE0
Locus tag GSY73_RS20245 Protein ID WP_023322192.1
Coordinates 4104037..4104324 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSY73_RS20215 4099673..4099921 + 249 WP_008805539.1 DUF1158 domain-containing protein -
GSY73_RS20220 4099938..4100279 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
GSY73_RS20225 4100310..4101425 - 1116 WP_162493283.1 MBL fold metallo-hydrolase -
GSY73_RS20230 4101605..4102186 + 582 WP_012968754.1 TetR/AcrR family transcriptional regulator -
GSY73_RS20235 4102186..4102554 + 369 WP_008805536.1 MmcQ/YjbR family DNA-binding protein -
GSY73_RS20240 4102674..4103327 + 654 WP_012968755.1 oxygen-insensitive NAD(P)H nitroreductase -
GSY73_RS20245 4104037..4104324 - 288 WP_023322192.1 helix-turn-helix transcriptional regulator Antitoxin
GSY73_RS20250 4104317..4104634 - 318 WP_012542526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GSY73_RS20255 4104819..4105862 - 1044 WP_131024424.1 DUF2157 domain-containing protein -
GSY73_RS20260 4106392..4107258 - 867 WP_008805530.1 helix-turn-helix transcriptional regulator -
GSY73_RS20265 4107367..4108794 + 1428 WP_131024423.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-89)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T146055 WP_012542526.1 NZ_CP047360:c4104634-4104317 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T146055 NZ_CP062719:c3370047-3369673 [Escherichia coli O157:H7]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10801.48 Da        Isoelectric Point: 10.2212

>AT146055 WP_023322192.1 NZ_CP047360:c4104324-4104037 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANVTRASLDTLTR
YALACGIKHPQILLY

Download         Length: 288 bp

>AT146055 NZ_CP062719:c3370468-3370094 [Escherichia coli O157:H7]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B7GEF2

Antitoxin

Source ID Structure
AlphaFold DB A0A6H0BIE0

References