Detailed information of TA system TA146030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3391053..3391868
• Replicon: chromosome
• Accession: NZ_CP047349
• Organism: Proteus terrae subsp. cibarius strain ZN2

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: GTH25_RS15590
• Protein ID: WP_075673429.1
• Coordinates: 3391395..3391868 (+)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: GTH25_RS15585
• Protein ID: WP_075673430.1
• Coordinates: 3391053..3391388 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH25_RS15565	3386742..3388166	+	1425	WP_072069056.1	aspartate ammonia-lyase	-
GTH25_RS15570	3388325..3389650	+	1326	WP_036934359.1	anaerobic C4-dicarboxylate transporter	-
GTH25_RS15575	3389834..3390406	+	573	WP_075673431.1	transcriptional regulator	-
GTH25_RS15585	3391053..3391388	+	336	WP_075673430.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
GTH25_RS15590	3391395..3391868	+	474	WP_075673429.1	type II toxin-antitoxin system YhaV family toxin	Toxin
GTH25_RS15595	3392179..3392826	+	648	WP_164530724.1	CPBP family intramembrane metalloprotease	-
GTH25_RS15600	3393129..3393764	+	636	WP_075673427.1	DUF1449 family protein	-
GTH25_RS15605	3393851..3396046	+	2196	WP_099659727.1	flotillin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-147)

Antitoxin

• PrlF antitoxin (15-109)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 18639.26 Da
• Isoelectric Point: 9.2715

>T146030 WP_075673429.1 NZ_CP047349:3391395-3391868 [Proteus terrae subsp. cibarius]
MDFPTDLNGWTIYAHPCFKQQYDELLQKVEALKKKYPEDYQKKADTKIFAHVLKSIVNITNDPRAPEFRLGNTLGEENKN
WSRIKLVNGRYRLFFRFSFKEKIIILAWINDNDTLRTYGNKTDAYRVFEKKLKKGHPPKNWNDLFSDCINEADCQDP

Nucleotide

• Length: 474 bp

>T146030 NZ_CP062717:4242572-4242790 [Escherichia coli O157:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12312.67 Da
• Isoelectric Point: 4.2560

>AT146030 WP_075673430.1 NZ_CP047349:3391053-3391388 [Proteus terrae subsp. cibarius]
MLTHVCTDNIALEADSKITERHQTTIPASIREALHLSGGDRIHYKLLSTGDVLISRHSDGSEDPVMASFLNFLAQDITNH
PQTIQPLDISRGFELVEGMDINLDDDITDNE

Nucleotide

• Length: 336 bp

>AT146030 NZ_CP062717:4242172-4242546 [Escherichia coli O157:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG