Detailed information of TA system TA146028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3293979..3294577
• Replicon: chromosome
• Accession: NZ_CP047349
• Organism: Proteus terrae subsp. cibarius strain ZN2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GTH25_RS15150
• Protein ID: WP_164530712.1
• Coordinates: 3293979..3294302 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTH25_RS15155
• Protein ID: WP_000058216.1
• Coordinates: 3294299..3294577 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH25_RS15120	3289692..3290078	-	387	WP_164530709.1	hypothetical protein	-
GTH25_RS15125	3290075..3290647	-	573	WP_164530710.1	type IV conjugative transfer system lipoprotein TraV	-
GTH25_RS15130	3290722..3292011	-	1290	WP_164530711.1	TraB/VirB10 family protein	-
GTH25_RS15135	3292014..3292910	-	897	WP_164530816.1	type-F conjugative transfer system secretin TraK	-
GTH25_RS15140	3292894..3293520	-	627	WP_025441422.1	pilus assembly protein	-
GTH25_RS15145	3293517..3293798	-	282	WP_000433891.1	type IV conjugative transfer system protein TraL	-
GTH25_RS15150	3293979..3294302	+	324	WP_164530712.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTH25_RS15155	3294299..3294577	+	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
GTH25_RS15160	3294603..3295238	-	636	WP_164530713.1	DUF4400 domain-containing protein	-
GTH25_RS15165	3295225..3295785	-	561	WP_164530714.1	conjugative transfer protein	-
GTH25_RS15170	3295795..3297615	-	1821	WP_164530715.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(H) / dfrA1	-	3170091..3311563	141472

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12304.36 Da
• Isoelectric Point: 9.7659

>T146028 WP_164530712.1 NZ_CP047349:3293979-3294302 [Proteus terrae subsp. cibarius]
MSWKIDFYDGVEEQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAHEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T146028 NZ_CP062717:3620169-3620456 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT146028 WP_000058216.1 NZ_CP047349:3294299-3294577 [Proteus terrae subsp. cibarius]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT146028 NZ_CP062717:3619978-3620169 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA