Detailed information of TA system TA146022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4058912..4059414
• Replicon: chromosome
• Accession: NZ_CP047344
• Organism: Proteus vulgaris strain ZN3

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A6G6SM64
• Locus tag: GTH24_RS19000
• Protein ID: WP_072068852.1
• Coordinates: 4059160..4059414 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: GTH24_RS18995
• Protein ID: WP_072068851.1
• Coordinates: 4058912..4059163 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH24_RS18965	4054342..4055076	+	735	WP_072068845.1	phosphate signaling complex protein PhoU	-
GTH24_RS18970	4055292..4056131	+	840	WP_072068846.1	ABC transporter substrate-binding protein	-
GTH24_RS18975	4056239..4056976	+	738	WP_072068847.1	amino acid ABC transporter permease	-
GTH24_RS18980	4056978..4057724	+	747	WP_164526835.1	amino acid ABC transporter permease	-
GTH24_RS18985	4057975..4058379	-	405	WP_072068849.1	DUF1240 domain-containing protein	-
GTH24_RS18990	4058382..4058786	-	405	WP_072068850.1	DUF1240 domain-containing protein	-
GTH24_RS18995	4058912..4059163	+	252	WP_072068851.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
GTH24_RS19000	4059160..4059414	+	255	WP_072068852.1	Txe/YoeB family addiction module toxin	Toxin
GTH24_RS19005	4059571..4060929	+	1359	WP_072068853.1	NCS2 family permease	-
GTH24_RS19010	4061009..4061320	+	312	WP_072068854.1	monooxygenase	-
GTH24_RS19015	4061370..4061918	-	549	WP_072068855.1	GNAT family N-acetyltransferase	-
GTH24_RS19020	4061921..4063393	-	1473	WP_072068856.1	glycosyltransferase family 39 protein	-
GTH24_RS19025	4063582..4064079	+	498	WP_072068857.1	Der GTPase-activating protein YihI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10226.86 Da
• Isoelectric Point: 9.9664

>T146022 WP_072068852.1 NZ_CP047344:4059160-4059414 [Proteus vulgaris]
MKLIFSEQSWSDYLYWQQMDKRIIKRINELIKDIKRTPFSGIGKPEPLKHNLAGFWSRRITDEHRLIYRITDPAIEIASC
RYHY

Nucleotide

• Length: 255 bp

>T146022 NZ_CP062717:c2891922-2891746 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9463.77 Da
• Isoelectric Point: 4.4354

>AT146022 WP_072068851.1 NZ_CP047344:4058912-4059163 [Proteus vulgaris]
MKTINYTEARQNLTSIMNETIDDRVPIMITRQKGIPCVLLSLDEYQALEETAYLLRSPANARHLLDSIDELNQGKGIEKD
ITE

Nucleotide

• Length: 252 bp

>AT146022 NZ_CP062717:c2891700-2891284 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A6G6SM64