Detailed information of TA system TA146010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1052890..1053485
• Replicon: chromosome
• Accession: NZ_CP047340
• Organism: Proteus terrae subsp. cibarius strain ZF1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GTH23_RS04725
• Protein ID: WP_156733604.1
• Coordinates: 1052890..1053168 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GTH23_RS04730
• Protein ID: WP_156733602.1
• Coordinates: 1053168..1053485 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTH23_RS04700	1048362..1048826	+	465	WP_156733609.1	GNAT family N-acetyltransferase	-
GTH23_RS04705	1048984..1049337	-	354	WP_088495238.1	flagella biosynthesis regulatory protein FliT	-
GTH23_RS04710	1049356..1049754	-	399	WP_023581844.1	flagellar export chaperone FliS	-
GTH23_RS04715	1049775..1051193	-	1419	WP_156733607.1	flagellar filament capping protein FliD	-
GTH23_RS04720	1051581..1052663	+	1083	WP_156733606.1	FliC/FljB family flagellin	-
GTH23_RS04725	1052890..1053168	+	279	WP_156733604.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GTH23_RS04730	1053168..1053485	+	318	WP_156733602.1	HigA family addiction module antidote protein	Antitoxin
GTH23_RS04735	1053861..1054982	+	1122	WP_156733600.1	FliC/FljB family flagellin	-
GTH23_RS04740	1055425..1056147	+	723	WP_036912728.1	RNA polymerase sigma factor FliA	-
GTH23_RS04745	1056206..1056736	+	531	WP_088495242.1	flagella biosynthesis regulatory protein FliZ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10815.32 Da
• Isoelectric Point: 9.3592

>T146010 WP_156733604.1 NZ_CP047340:1052890-1053168 [Proteus terrae subsp. cibarius]
MIKSFGHKGLKQLFEKGITSGVLAQDVERINDRLQAIDVAQNISELNRQIYKLHKLKGEREGYWSITVRANWRITFQFIN
GDAYILNYEDYH

Nucleotide

• Length: 279 bp

>T146010 NZ_CP062716:74039-74197 [Escherichia coli O157:H7]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATAGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11565.27 Da
• Isoelectric Point: 7.0104

>AT146010 WP_156733602.1 NZ_CP047340:1053168-1053485 [Proteus terrae subsp. cibarius]
MKQFKISHPGEIIARDLADMGISGRRFALNIGVTPATVSRLLTGKTALTPSLAIRIAAALGSTPEFWLRLQSIYDLRQLE
DQIDTSKIEVYGEVADLVDRQSYSS

Nucleotide

• Length: 318 bp

>AT146010 NZ_CP062716:73776-73974 [Escherichia coli O157:H7]
TCACACGGATTGCCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAATGTGGTCAGCGTGCTGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC