Detailed information of TA system TA145954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 420837..421354
• Replicon: chromosome
• Accession: NZ_CP047321
• Organism: Staphylococcus aureus strain RJ1267

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: GSF72_RS02005
• Protein ID: WP_000113262.1
• Coordinates: 421088..421354 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: GSF72_RS02000
• Protein ID: WP_000584499.1
• Coordinates: 420837..421088 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSF72_RS01960	416303..416515	+	213	Protein_385	cystatin-like fold lipoprotein	-
GSF72_RS01965	416565..417239	-	675	WP_031919200.1	IS6-like element IS257 family transposase	-
GSF72_RS01975	418007..418564	+	558	WP_048665604.1	hypothetical protein	-
GSF72_RS01980	419048..419722	-	675	WP_001105987.1	IS6-like element IS257 family transposase	-
GSF72_RS01985	419770..419946	+	177	Protein_389	cystatin-like fold lipoprotein	-
GSF72_RS01990	419965..420564	+	600	WP_000162813.1	DsbA family protein	-
GSF72_RS01995	420571..420672	+	102	WP_001791744.1	hypothetical protein	-
GSF72_RS02000	420837..421088	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GSF72_RS02005	421088..421354	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
GSF72_RS02010	421389..421460	+	72	Protein_394	hypothetical protein	-
GSF72_RS02015	421649..423199	+	1551	WP_000727762.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
GSF72_RS02020	423385..423852	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
GSF72_RS02025	423936..424118	+	183	WP_000230294.1	hypothetical protein	-
GSF72_RS02030	424335..425159	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
GSF72_RS02035	425392..425928	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T145954 WP_000113262.1 NZ_CP047321:421088-421354 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T145954 NZ_CP062713:366677-366952 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT145954 WP_000584499.1 NZ_CP047321:420837-421088 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT145954 NZ_CP062713:366949-367308 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60