Detailed information of TA system TA145769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 109740..110397
• Replicon: plasmid pCf4128
• Accession: NZ_CP047280
• Organism: Citrobacter freundii strain N18-04128

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: F3113_RS25910
• Protein ID: WP_000270043.1
• Coordinates: 110047..110397 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F3113_RS25905
• Protein ID: WP_000124640.1
• Coordinates: 109740..110042 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3113_RS25860	105365..105793	+	429	WP_000591074.1	hypothetical protein	-
F3113_RS25865	105850..106209	+	360	WP_000422768.1	hypothetical protein	-
F3113_RS25870	106209..106655	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
F3113_RS25875	106652..107170	+	519	WP_000210756.1	nitrite reductase	-
F3113_RS25880	107170..107400	+	231	WP_117027707.1	hypothetical protein	-
F3113_RS25885	107387..108244	+	858	WP_001167032.1	hypothetical protein	-
F3113_RS25890	108475..109002	+	528	WP_001236377.1	thermonuclease family protein	-
F3113_RS25895	109060..109332	+	273	WP_001043047.1	HU family DNA-binding protein	-
F3113_RS25900	109420..109713	+	294	WP_001239997.1	chromosome segregation protein ParM	-
F3113_RS25905	109740..110042	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
F3113_RS25910	110047..110397	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F3113_RS25915	110560..111108	+	549	WP_001061574.1	transcriptional regulator	-
F3113_RS25920	111449..111643	+	195	WP_000343597.1	hypothetical protein	-
F3113_RS25925	111654..112025	+	372	WP_000516916.1	hypothetical protein	-
F3113_RS25930	112018..112488	+	471	WP_001281821.1	hypothetical protein	-
F3113_RS25935	112503..112838	-	336	WP_000683476.1	hypothetical protein	-
F3113_RS25940	112935..113423	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
F3113_RS25945	113614..114876	+	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / blaCMY-4 / blaOXA-204 / aph(3'')-Ib / aph(6)-Id / tet(A) / catA2 / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	1..262422	262422

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T145769 WP_000270043.1 NZ_CP047280:c110397-110047 [Citrobacter freundii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T145769 NZ_CP062469:918210-918572 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATACAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT145769 WP_000124640.1 NZ_CP047280:c110042-109740 [Citrobacter freundii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT145769 NZ_CP062469:918043-918213 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9