Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 385856..386522 Replicon chromosome
Accession NZ_CP047279
Organism Citrobacter freundii strain N18-04128

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag F3113_RS01810 Protein ID WP_003847996.1
Coordinates 386205..386522 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag F3113_RS01805 Protein ID WP_003837894.1
Coordinates 385856..386152 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3113_RS01790 383032..383505 - 474 WP_003023524.1 transcription elongation factor GreB -
F3113_RS01795 383731..384450 + 720 WP_001157751.1 two-component system response regulator OmpR -
F3113_RS01800 384447..385799 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
F3113_RS01805 385856..386152 - 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
F3113_RS01810 386205..386522 - 318 WP_003847996.1 hypothetical protein Toxin
F3113_RS01815 386645..388267 - 1623 WP_044701411.1 phosphoenolpyruvate carboxykinase (ATP) -
F3113_RS01820 388646..390364 + 1719 WP_183273576.1 DUF4153 domain-containing protein -
F3113_RS01825 390474..391352 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T145754 WP_003847996.1 NZ_CP047279:c386522-386205 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T145754 NZ_CP062467:1251598-1251705 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT145754 WP_003837894.1 NZ_CP047279:c386152-385856 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT145754 NZ_CP062467:c1251782-1251722 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References