Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3697736..3698277 Replicon chromosome
Accession NZ_CP047277
Organism Escherichia coli strain N18-04097

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag F3052_RS17895 Protein ID WP_000615983.1
Coordinates 3697999..3698277 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag F3052_RS17890 Protein ID WP_000729703.1
Coordinates 3697736..3697996 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3052_RS17865 3692832..3694544 + 1713 Protein_3504 flagellar biosynthesis protein FlhA -
F3052_RS17870 3694768..3695265 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
F3052_RS17875 3695441..3696001 - 561 Protein_3506 C40 family peptidase -
F3052_RS17880 3696090..3697318 + 1229 WP_088895425.1 IS3-like element IS2 family transposase -
F3052_RS17885 3697329..3697526 - 198 Protein_3508 peptidoglycan endopeptidase -
F3052_RS17890 3697736..3697996 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
F3052_RS17895 3697999..3698277 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
F3052_RS17900 3698433..3699173 + 741 WP_001225679.1 murein L,D-transpeptidase -
F3052_RS17905 3699144..3699911 - 768 WP_000333380.1 class II glutamine amidotransferase -
F3052_RS17910 3700117..3700695 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T145745 WP_000615983.1 NZ_CP047277:3697999-3698277 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T145745 NZ_CP062465:870847-871209 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATACAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT145745 WP_000729703.1 NZ_CP047277:3697736-3697996 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT145745 NZ_CP062465:870680-870850 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References