145744

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3690203..3690897 Replicon chromosome
Accession NZ_CP047277
Organism Escherichia coli strain N18-04097

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag F3052_RS17845 Protein ID WP_001263489.1
Coordinates 3690203..3690601 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag F3052_RS17850 Protein ID WP_000554758.1
Coordinates 3690604..3690897 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3685791..3685871 - 81 NuclAT_12 - -
- 3685791..3685871 - 81 NuclAT_12 - -
- 3685791..3685871 - 81 NuclAT_12 - -
- 3685791..3685871 - 81 NuclAT_12 - -
F3052_RS17820 3686467..3686925 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
F3052_RS17825 3687186..3688643 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
F3052_RS17830 3688700..3689221 - 522 Protein_3497 peptide chain release factor H -
F3052_RS17835 3689217..3689423 - 207 Protein_3498 RtcB family protein -
F3052_RS17840 3689741..3690193 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
F3052_RS17845 3690203..3690601 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
F3052_RS17850 3690604..3690897 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
F3052_RS17855 3690949..3692004 - 1056 WP_001226164.1 DNA polymerase IV -
F3052_RS17860 3692075..3692860 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
F3052_RS17865 3692832..3694544 + 1713 Protein_3504 flagellar biosynthesis protein FlhA -
F3052_RS17870 3694768..3695265 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T145744 WP_001263489.1 NZ_CP047277:c3690601-3690203 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T145744 NZ_CP062465:851242-851451 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT145744 WP_000554758.1 NZ_CP047277:c3690897-3690604 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT145744 NZ_CP062465:850937-851230 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References