Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 609925..610724 Replicon chromosome
Accession NZ_CP047277
Organism Escherichia coli strain N18-04097

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag F3052_RS02935 Protein ID WP_000347273.1
Coordinates 609925..610389 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag F3052_RS02940 Protein ID WP_001307405.1
Coordinates 610389..610724 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3052_RS02905 604926..605360 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
F3052_RS02910 605378..606256 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
F3052_RS02915 606246..607025 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
F3052_RS02920 607036..607509 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
F3052_RS02925 607532..608812 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
F3052_RS02930 609061..609870 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
F3052_RS02935 609925..610389 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
F3052_RS02940 610389..610724 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
F3052_RS02945 610873..612444 - 1572 WP_097725156.1 galactarate dehydratase -
F3052_RS02950 612819..614153 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
F3052_RS02955 614169..614939 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 609925..633004 23079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T145726 WP_000347273.1 NZ_CP047277:c610389-609925 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T145726 NZ_CP062421:2299505-2299612 [Staphylococcus aureus]
ATCTTCAATTTATTAATTGACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCAAATTGGTTACGAAC
GCGCAGCAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT145726 WP_001307405.1 NZ_CP047277:c610724-610389 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT145726 NZ_CP062421:c2299689-2299629 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References