Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 109740..110397 Replicon plasmid pCf4085
Accession NZ_CP047276
Organism Citrobacter freundii strain N18-04085

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag F3084_RS25750 Protein ID WP_000270043.1
Coordinates 110047..110397 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag F3084_RS25745 Protein ID WP_000124640.1
Coordinates 109740..110042 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3084_RS25700 105365..105793 + 429 WP_000591074.1 hypothetical protein -
F3084_RS25705 105850..106209 + 360 WP_000422768.1 hypothetical protein -
F3084_RS25710 106209..106655 + 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -
F3084_RS25715 106652..107170 + 519 WP_000210756.1 nitrite reductase -
F3084_RS25720 107170..107400 + 231 WP_117027707.1 hypothetical protein -
F3084_RS25725 107387..108244 + 858 WP_001167032.1 hypothetical protein -
F3084_RS25730 108475..109002 + 528 WP_001236377.1 thermonuclease family protein -
F3084_RS25735 109060..109332 + 273 WP_001043047.1 HU family DNA-binding protein -
F3084_RS25740 109420..109713 + 294 WP_001239997.1 chromosome segregation protein ParM -
F3084_RS25745 109740..110042 - 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
F3084_RS25750 110047..110397 - 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F3084_RS25755 110560..111108 + 549 WP_001061574.1 transcriptional regulator -
F3084_RS25760 111449..111643 + 195 WP_000343597.1 hypothetical protein -
F3084_RS25765 111654..112025 + 372 WP_000516916.1 hypothetical protein -
F3084_RS25770 112018..112488 + 471 WP_001281821.1 hypothetical protein -
F3084_RS25775 112503..112838 - 336 WP_000683476.1 hypothetical protein -
F3084_RS25780 112935..113456 + 522 WP_181937576.1 DUF1643 domain-containing protein -
F3084_RS25785 113609..114871 + 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / blaCMY-4 / blaOXA-204 / aph(3'')-Ib / aph(6)-Id / tet(A) / catA2 / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr - 1..282258 282258


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T145723 WP_000270043.1 NZ_CP047276:c110397-110047 [Citrobacter freundii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T145723 NZ_CP062421:c168085-167990 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT145723 WP_000124640.1 NZ_CP047276:c110042-109740 [Citrobacter freundii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT145723 NZ_CP062421:167905-167962 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9

Antitoxin

Source ID Structure

References