Detailed information of TA system TA145702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 120074..120731
• Replicon: plasmid pCf4078
• Accession: NZ_CP047274
• Organism: Citrobacter freundii strain N18-04078

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: F3085_RS26005
• Protein ID: WP_000270043.1
• Coordinates: 120381..120731 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F3085_RS26000
• Protein ID: WP_000124640.1
• Coordinates: 120074..120376 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3085_RS25955	115699..116127	+	429	WP_000591074.1	hypothetical protein	-
F3085_RS25960	116184..116543	+	360	WP_000422768.1	hypothetical protein	-
F3085_RS25965	116543..116989	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
F3085_RS25970	116986..117504	+	519	WP_000210756.1	nitrite reductase	-
F3085_RS25975	117504..117734	+	231	WP_117027707.1	hypothetical protein	-
F3085_RS25980	117721..118578	+	858	WP_001167032.1	hypothetical protein	-
F3085_RS25985	118809..119336	+	528	WP_001236377.1	thermonuclease family protein	-
F3085_RS25990	119394..119666	+	273	WP_001043047.1	HU family DNA-binding protein	-
F3085_RS25995	119754..120047	+	294	WP_001239997.1	chromosome segregation protein ParM	-
F3085_RS26000	120074..120376	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
F3085_RS26005	120381..120731	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F3085_RS26010	120894..121442	+	549	WP_001061574.1	transcriptional regulator	-
F3085_RS26015	121783..121977	+	195	WP_000343597.1	hypothetical protein	-
F3085_RS26020	121988..122359	+	372	WP_000516916.1	hypothetical protein	-
F3085_RS26025	122352..122822	+	471	WP_001281821.1	hypothetical protein	-
F3085_RS26030	122837..123172	-	336	WP_000683476.1	hypothetical protein	-
F3085_RS26035	123269..123790	+	522	WP_181937576.1	DUF1643 domain-containing protein	-
F3085_RS26040	123943..125205	+	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / blaCMY-4 / blaOXA-204 / catA2 / blaTEM-1B / aac(3)-IId / mph(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr	-	1..277055	277055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T145702 WP_000270043.1 NZ_CP047274:c120731-120381 [Citrobacter freundii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T145702 NZ_CP062419:18831-19007 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT145702 WP_000124640.1 NZ_CP047274:c120376-120074 [Citrobacter freundii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT145702 NZ_CP062419:c19169-18995 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9