Detailed information of TA system TA145681

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 119888..120545
• Replicon: plasmid pKp3892
• Accession: NZ_CP047272
• Organism: Klebsiella pneumoniae strain N16-03892

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: F3090_RS26820
• Protein ID: WP_000270043.1
• Coordinates: 120195..120545 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F3090_RS26815
• Protein ID: WP_000124640.1
• Coordinates: 119888..120190 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3090_RS26770	115513..115941	+	429	WP_000591074.1	hypothetical protein	-
F3090_RS26775	115998..116357	+	360	WP_000422768.1	hypothetical protein	-
F3090_RS26780	116357..116803	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
F3090_RS26785	116800..117318	+	519	WP_000210756.1	nitrite reductase	-
F3090_RS26790	117318..117548	+	231	WP_117027707.1	hypothetical protein	-
F3090_RS26795	117535..118392	+	858	WP_001167032.1	hypothetical protein	-
F3090_RS26800	118623..119150	+	528	WP_001236377.1	thermonuclease family protein	-
F3090_RS26805	119208..119480	+	273	WP_001043047.1	HU family DNA-binding protein	-
F3090_RS26810	119568..119861	+	294	WP_001239997.1	chromosome segregation protein ParM	-
F3090_RS26815	119888..120190	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
F3090_RS26820	120195..120545	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F3090_RS26825	120708..121256	+	549	WP_001061574.1	transcriptional regulator	-
F3090_RS26830	121597..121791	+	195	WP_000343597.1	hypothetical protein	-
F3090_RS26835	121802..122173	+	372	WP_000516916.1	hypothetical protein	-
F3090_RS26840	122166..122636	+	471	WP_001281821.1	hypothetical protein	-
F3090_RS26845	122651..122986	-	336	WP_000683476.1	hypothetical protein	-
F3090_RS26850	123083..123604	+	522	WP_181937576.1	DUF1643 domain-containing protein	-
F3090_RS26855	123757..125019	+	1263	WP_000608644.1	IS1380-like element ISEc9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / blaCMY-4 / blaOXA-204 / aph(3'')-Ib / aph(6)-Id / tet(A) / catA2 / blaTEM-1B / aac(3)-IId	-	1..271744	271744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T145681 WP_000270043.1 NZ_CP047272:c120545-120195 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T145681 NZ_CP062416:2650666-2650875 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT145681 WP_000124640.1 NZ_CP047272:c120190-119888 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT145681 NZ_CP062416:2650361-2650654 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9