Detailed information of TA system TA145663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 361937..362583
• Replicon: chromosome
• Accession: NZ_CP047271
• Organism: Klebsiella pneumoniae strain N16-03892

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: F3090_RS01700
• Protein ID: WP_023317328.1
• Coordinates: 361937..362284 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: F3090_RS01705
• Protein ID: WP_032412314.1
• Coordinates: 362284..362583 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F3090_RS01690	357863..359296	+	1434	WP_002920564.1	glycogen synthase GlgA	-
F3090_RS01695	359314..361761	+	2448	WP_002920561.1	glycogen phosphorylase	-
F3090_RS01700	361937..362284	+	348	WP_023317328.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F3090_RS01705	362284..362583	+	300	WP_032412314.1	XRE family transcriptional regulator	Antitoxin
F3090_RS01710	362646..364154	-	1509	WP_032412313.1	glycerol-3-phosphate dehydrogenase	-
F3090_RS01715	364359..364688	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
F3090_RS01720	364739..365569	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
F3090_RS01725	365619..366377	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13562.60 Da
• Isoelectric Point: 6.2327

>T145663 WP_023317328.1 NZ_CP047271:361937-362284 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
ILCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T145663 NZ_CP062415:22041-22217 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10828.64 Da
• Isoelectric Point: 10.1464

>AT145663 WP_032412314.1 NZ_CP047271:362284-362583 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGIKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT145663 NZ_CP062415:c22379-22205 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG