Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 417846..418512 Replicon chromosome
Accession NZ_CP047269
Organism Citrobacter freundii strain N16-03880

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5Q945
Locus tag F3114_RS02025 Protein ID WP_003847996.1
Coordinates 418195..418512 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4U6IRD5
Locus tag F3114_RS02020 Protein ID WP_003837894.1
Coordinates 417846..418142 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3114_RS02005 415022..415495 - 474 WP_003023524.1 transcription elongation factor GreB -
F3114_RS02010 415721..416440 + 720 WP_001157751.1 two-component system response regulator OmpR -
F3114_RS02015 416437..417789 + 1353 WP_003837891.1 two-component system sensor histidine kinase EnvZ -
F3114_RS02020 417846..418142 - 297 WP_003837894.1 helix-turn-helix domain-containing protein Antitoxin
F3114_RS02025 418195..418512 - 318 WP_003847996.1 hypothetical protein Toxin
F3114_RS02030 418635..420257 - 1623 WP_044701411.1 phosphoenolpyruvate carboxykinase (ATP) -
F3114_RS02035 420636..422354 + 1719 WP_183273576.1 DUF4153 domain-containing protein -
F3114_RS02040 422464..423342 - 879 WP_003023531.1 Hsp33 family molecular chaperone HslO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12225.15 Da        Isoelectric Point: 10.0909

>T145644 WP_003847996.1 NZ_CP047269:c418512-418195 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSNMLI

Download         Length: 318 bp

>T145644 NZ_CP062413:c216538-216443 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11157.93 Da        Isoelectric Point: 7.2050

>AT145644 WP_003837894.1 NZ_CP047269:c418142-417846 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Download         Length: 297 bp

>AT145644 NZ_CP062413:216358-216415 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5Q945

Antitoxin

Source ID Structure
AlphaFold DB A0A4U6IRD5

References