Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 34641..35289 Replicon chromosome
Accession NZ_CP047269
Organism Citrobacter freundii strain N16-03880

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag F3114_RS00170 Protein ID WP_016151211.1
Coordinates 34641..35003 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D7LEH2
Locus tag F3114_RS00175 Protein ID WP_003840660.1
Coordinates 34990..35289 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3114_RS00155 31100..32428 + 1329 WP_003023927.1 MFS transporter -
F3114_RS00160 32571..33962 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
F3114_RS00165 34088..34540 + 453 WP_003023933.1 DUF1198 family protein -
F3114_RS00170 34641..35003 + 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F3114_RS00175 34990..35289 + 300 WP_003840660.1 helix-turn-helix transcriptional regulator Antitoxin
F3114_RS00180 35408..36601 + 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
F3114_RS00185 36650..38032 - 1383 WP_016151210.1 glycoside hydrolase family 1 protein -
F3114_RS00190 38127..38420 - 294 WP_003840665.1 hypothetical protein -
F3114_RS00195 38551..38967 + 417 WP_003023951.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T145641 WP_016151211.1 NZ_CP047269:34641-35003 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

>T145641 NZ_CP062413:37699-37950 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11016.85 Da        Isoelectric Point: 8.5047

>AT145641 WP_003840660.1 NZ_CP047269:34990-35289 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp

>AT145641 NZ_CP062413:37940-38113 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGATGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LEH2

References