Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5790369..5790950 Replicon chromosome
Accession NZ_CP047265
Organism Pseudomonas asturiensis strain CC1524

Toxin (Protein)


Gene name graT Uniprot ID A0A7Z2LHL1
Locus tag N015_RS25975 Protein ID WP_032610550.1
Coordinates 5790684..5790950 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A7Z2LH90
Locus tag N015_RS25970 Protein ID WP_024687447.1
Coordinates 5790369..5790674 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N015_RS25940 5786073..5786765 + 693 WP_024687452.1 HAD-IA family hydrolase -
N015_RS25945 5786847..5787179 - 333 WP_024687451.1 hypothetical protein -
N015_RS25950 5787278..5787703 - 426 WP_024687450.1 secondary thiamine-phosphate synthase enzyme YjbQ -
N015_RS25955 5787947..5789284 - 1338 WP_024687449.1 ammonium transporter -
N015_RS25960 5789320..5789658 - 339 WP_002555808.1 P-II family nitrogen regulator -
N015_RS26490 5789855..5789998 - 144 WP_080266083.1 hypothetical protein -
N015_RS25965 5790079..5790345 + 267 WP_024687448.1 accessory factor UbiK family protein -
N015_RS25970 5790369..5790674 - 306 WP_024687447.1 HigA family addiction module antidote protein Antitoxin
N015_RS25975 5790684..5790950 - 267 WP_032610550.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N015_RS25980 5791125..5791739 - 615 WP_200868022.1 hypothetical protein -
N015_RS25985 5792884..5793690 - 807 WP_152535035.1 SEL1-like repeat protein -
N015_RS25990 5794254..5794772 + 519 Protein_5095 Bro-N domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-88)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-91)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10042.56 Da        Isoelectric Point: 10.2055

>T145636 WP_032610550.1 NZ_CP047265:c5790950-5790684 [Pseudomonas asturiensis]
MLTGRPKGLFQNGRTRLWPALRMVAERKLAMLDAAVLLSDLRSPPGNRLETLDGNRRGQHSIRINAQFRICFVWGENGPE
DVEIVDYH

Download         Length: 267 bp

>T145636 NZ_CP062412:2336828-2337094 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11172.98 Da        Isoelectric Point: 7.1063

>AT145636 WP_024687447.1 NZ_CP047265:c5790674-5790369 [Pseudomonas asturiensis]
MTKNGMRPVHPGEVLKEEYLEPMGLTAAALARALNVSTPTVNDIVLQRRGVSADVALRLGVCLDTSPEFWLNLQLTYDLR
KAEIEKGAQIRDQIRCLAHCA

Download         Length: 306 bp

>AT145636 NZ_CP062412:2336577-2336828 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z2LHL1

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z2LH90

References