Detailed information of TA system TA145606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3006899..3007592
• Replicon: chromosome
• Accession: NZ_CP047231
• Organism: Escherichia coli strain BL21-AI

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: GSU80_RS14515
• Protein ID: WP_000415584.1
• Coordinates: 3007296..3007592 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: GSU80_RS14510
• Protein ID: WP_000650107.1
• Coordinates: 3006899..3007294 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSU80_RS14500	3002763..3005021	-	2259	WP_001281877.1	DNA topoisomerase IV subunit A	-
GSU80_RS14505	3005159..3006766	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
GSU80_RS14510	3006899..3007294	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
GSU80_RS14515	3007296..3007592	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
GSU80_RS14520	3007797..3008279	-	483	WP_000183505.1	transcriptional regulator	-
GSU80_RS14525	3008332..3008724	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
GSU80_RS14530	3008876..3009535	+	660	WP_001221493.1	two-component system response regulator QseB	-
GSU80_RS14535	3009532..3010881	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
GSU80_RS14540	3010927..3011259	-	333	WP_000917684.1	DUF2645 family protein	-
GSU80_RS14545	3011578..3012159	+	582	WP_000072420.1	NADPH:quinone oxidoreductase MdaB	-
GSU80_RS14550	3012190..3012504	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T145606 WP_000415584.1 NZ_CP047231:c3007592-3007296 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T145606 NZ_CP062409:2661216-2661578 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT145606 WP_000650107.1 NZ_CP047231:c3007294-3006899 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT145606 NZ_CP062409:2661049-2661219 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65