Detailed information of TA system TA145590

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1370335..1370706
• Replicon: chromosome
• Accession: NZ_CP047231
• Organism: Escherichia coli strain BL21-AI

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: GSU80_RS06705
• Protein ID: WP_001317028.1
• Coordinates: 1370512..1370706 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1370335..1370513 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSU80_RS06680	1366290..1367663	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
GSU80_RS06685	1367792..1368727	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
GSU80_RS06690	1368779..1370014	-	1236	WP_000040858.1	site-specific integrase	-
GSU80_RS06695	1370016..1370231	-	216	WP_000079604.1	excisionase XisR	-
GSU80_RS06700	1370310..1370519	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1370335..1370513	+	179	NuclAT_0	-	Antitoxin
-	1370335..1370513	+	179	NuclAT_0	-	Antitoxin
-	1370335..1370513	+	179	NuclAT_0	-	Antitoxin
-	1370335..1370513	+	179	NuclAT_0	-	Antitoxin
GSU80_RS06705	1370512..1370706	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
GSU80_RS06710	1370763..1371572	-	810	WP_000166319.1	recombination protein RecT	-
GSU80_RS06715	1371565..1372664	-	1100	Protein_1308	PD-(D/E)XK nuclease-like domain-containing protein	-
GSU80_RS06720	1372766..1373041	-	276	WP_000632297.1	protein RacC	-
GSU80_RS06725	1373116..1373286	-	171	WP_001352098.1	conserved protein, Rac prophage	-
GSU80_RS06730	1373286..1373507	-	222	WP_000560223.1	killing protein KilR	-
GSU80_RS06735	1373949..1374437	+	489	WP_001312793.1	superinfection exclusion protein B	-
GSU80_RS06740	1374434..1374589	-	156	WP_001169151.1	YdaF family protein	-
GSU80_RS06745	1374733..1374945	+	213	WP_012775985.1	phage lysis protein EssD	-
GSU80_RS06750	1374950..1375294	+	345	WP_000193293.1	YdfR family protein	-
GSU80_RS06755	1375260..1375532	-	273	WP_000370546.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1361520..1399427	37907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T145590 WP_001317028.1 NZ_CP047231:c1370706-1370512 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T145590 NZ_CP062408:1209906-1210094 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT145590 NZ_CP047231:1370335-1370513 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin