145585

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 259017..259711 Replicon chromosome
Accession NZ_CP047231
Organism Escherichia coli strain BL21-AI

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag GSU80_RS01175 Protein ID WP_001263493.1
Coordinates 259313..259711 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag GSU80_RS01170 Protein ID WP_000554757.1
Coordinates 259017..259310 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU80_RS01150 254657..255154 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
GSU80_RS01155 255370..257082 - 1713 Protein_225 flagellar biosynthesis protein FlhA -
GSU80_RS01160 257054..257839 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
GSU80_RS01165 257910..258965 + 1056 WP_001226164.1 DNA polymerase IV -
GSU80_RS01170 259017..259310 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GSU80_RS01175 259313..259711 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GSU80_RS01180 259721..260173 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
GSU80_RS01185 260419..260622 + 204 Protein_231 RNA ligase RtcB family protein -
GSU80_RS01190 260621..261142 + 522 Protein_232 peptide chain release factor H -
GSU80_RS01195 261199..262656 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
GSU80_RS01200 262917..263375 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
GSU80_RS01205 263467..264711 + 1245 WP_000189541.1 esterase FrsA -
- 263971..264051 + 81 NuclAT_13 - -
- 263971..264051 + 81 NuclAT_13 - -
- 263971..264051 + 81 NuclAT_13 - -
- 263971..264051 + 81 NuclAT_13 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - IlpA / gmhA/lpcA 210415..265993 55578


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T145585 WP_001263493.1 NZ_CP047231:259313-259711 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T145585 NZ_CP062407:c2463462-2463367 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT145585 WP_000554757.1 NZ_CP047231:259017-259310 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT145585 NZ_CP062407:2463282-2463339 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References