Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4060..4652 Replicon plasmid p3
Accession NZ_CP047229
Organism Moraxella osloensis strain YV1

Toxin (Protein)


Gene name graT Uniprot ID A0A120KR99
Locus tag GSF12_RS12740 Protein ID WP_062335123.1
Coordinates 4060..4341 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A120KRC1
Locus tag GSF12_RS12745 Protein ID WP_062335121.1
Coordinates 4356..4652 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSF12_RS12710 639..866 - 228 WP_062335103.1 helix-turn-helix transcriptional regulator -
GSF12_RS12715 1040..1324 + 285 WP_159375883.1 hypothetical protein -
GSF12_RS12720 1384..1749 + 366 WP_159375884.1 hypothetical protein -
GSF12_RS12725 1868..2314 - 447 WP_159375885.1 hypothetical protein -
GSF12_RS12730 2662..3132 + 471 WP_159375886.1 plasmid mobilization relaxosome protein MobC -
GSF12_RS12735 3215..3724 + 510 WP_159375887.1 relaxase/mobilization nuclease domain-containing protein -
GSF12_RS12740 4060..4341 + 282 WP_062335123.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GSF12_RS12745 4356..4652 + 297 WP_062335121.1 HigA family addiction module antidote protein Antitoxin
GSF12_RS12750 4759..5921 + 1163 WP_159375888.1 IS3 family transposase -
GSF12_RS12755 6181..6510 - 330 WP_159375889.1 hypothetical protein -
GSF12_RS12760 6510..7271 - 762 WP_159375890.1 ParA family protein -
GSF12_RS12765 7433..7843 - 411 WP_159374790.1 IS200/IS605 family transposase -
GSF12_RS12770 7892..8995 + 1104 WP_159375891.1 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..26254 26254


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-92)

Antitoxin

(11-63)


Sequences


Toxin        


Download         Length: 94 a.a.        Molecular weight: 10798.23 Da        Isoelectric Point: 6.8610

>T145583 WP_062335123.1 NZ_CP047229:4060-4341 [Moraxella osloensis]
MIVNFACDKTKTLFTTGNTRHFSSIKKVAERKLLQLDNAITLEDLRSPPGNHLEALFGDREGQHSIRINDQWRICFVWTQ
EGPTEVEIVDYHR

Download         Length: 282 bp

>T145583 NZ_CP062407:2280537-2280857 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTTTATTTAAAAGAGTTCGATGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTA
A

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11046.78 Da        Isoelectric Point: 7.2020

>AT145583 WP_062335121.1 NZ_CP047229:4356-4652 [Moraxella osloensis]
MKNGMRPIHPGEILREDYLAPLEMSANALAKAIGVTPARINEIVREERGITADTALRLAKYFGGNAKFWLNLQTTYELRL
AETQDTHFLDKIMPLAFS

Download         Length: 297 bp

>AT145583 NZ_CP062407:2280325-2280534 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACTTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
CGAACACACAGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A120KR99


Antitoxin

Source ID Structure
AlphaFold DB A0A120KRC1

References