Detailed information of TA system TA145564

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132030..132283
• Replicon: plasmid unnamed2
• Accession: NZ_CP047194
• Organism: Klebsiella pneumoniae strain Kp36

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: GS419_RS28930
• Protein ID: WP_001312851.1
• Coordinates: 132030..132179 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	132224..132283 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS419_RS28895	127389..127804	-	416	Protein_167	IS1 family transposase	-
GS419_RS28900	128053..128454	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
GS419_RS28905	128387..128644	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
GS419_RS28910	128737..129390	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
GS419_RS28915	130329..131186	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
GS419_RS28920	131179..131253	-	75	WP_001375168.1	RepA leader peptide Tap	-
GS419_RS28925	131498..131746	-	249	WP_000083837.1	replication regulatory protein RepA	-
GS419_RS28930	132030..132179	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	132224..132283	+	60	NuclAT_1	-	Antitoxin
-	132224..132283	+	60	NuclAT_1	-	Antitoxin
-	132224..132283	+	60	NuclAT_1	-	Antitoxin
-	132224..132283	+	60	NuclAT_1	-	Antitoxin
GS419_RS28935	132484..132714	-	231	WP_001736714.1	hypothetical protein	-
GS419_RS28940	132878..133477	-	600	WP_032083981.1	hypothetical protein	-
GS419_RS28945	133863..134063	-	201	WP_015059022.1	hypothetical protein	-
GS419_RS28950	134195..134755	-	561	WP_000139328.1	fertility inhibition protein FinO	-
GS419_RS28955	134810..135556	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
GS419_RS28960	135576..135737	-	162	Protein_180	DNA helicase	-
GS419_RS28965	135801..136505	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65	-	1..142228	142228
	-	flank	IS/Tn	-	-	135801..136505	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T145564 WP_001312851.1 NZ_CP047194:c132179-132030 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T145564 NZ_CP062405:c2204217-2204110 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT145564 NZ_CP047194:132224-132283 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin