Detailed information of TA system TA145560

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93774..94200
• Replicon: plasmid unnamed2
• Accession: NZ_CP047194
• Organism: Klebsiella pneumoniae strain Kp36

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GS419_RS28670
• Protein ID: WP_001312861.1
• Coordinates: 93774..93932 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	93976..94200 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS419_RS28635	88829..89056	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
GS419_RS28640	89150..89836	-	687	WP_015059009.1	PAS domain-containing protein	-
GS419_RS28645	90027..90410	-	384	WP_000124981.1	relaxosome protein TraM	-
GS419_RS28650	90687..91334	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
GS419_RS28655	91631..92452	-	822	WP_001234445.1	DUF945 domain-containing protein	-
GS419_RS28660	92563..92859	-	297	WP_001272251.1	hypothetical protein	-
GS419_RS30250	93159..93536	+	378	Protein_123	hypothetical protein	-
GS419_RS28670	93774..93932	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	93976..94200	-	225	NuclAT_0	-	Antitoxin
-	93976..94200	-	225	NuclAT_0	-	Antitoxin
-	93976..94200	-	225	NuclAT_0	-	Antitoxin
-	93976..94200	-	225	NuclAT_0	-	Antitoxin
GS419_RS28675	94212..94931	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
GS419_RS28680	94928..95362	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GS419_RS28685	95431..97454	-	2024	Protein_127	ParB/RepB/Spo0J family partition protein	-
GS419_RS28690	97515..97748	-	234	WP_000006003.1	DUF905 family protein	-
GS419_RS28695	97806..98333	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65	-	1..142228	142228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T145560 WP_001312861.1 NZ_CP047194:c93932-93774 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T145560 NZ_CP062405:c1835778-1835416 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGTGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGTTCATCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT145560 NZ_CP047194:c94200-93976 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin