Detailed information of TA system TA145529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 457415..457982
• Replicon: chromosome
• Accession: NZ_CP047171
• Organism: Xylella fastidiosa subsp. fastidiosa strain IVIA5235

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q87EE4
• Locus tag: D1605_RS01865
• Protein ID: WP_004090695.1
• Coordinates: 457415..457696 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: D1605_RS01870
• Protein ID: WP_004085172.1
• Coordinates: 457707..457982 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1605_RS01840	453838..454557	-	720	Protein_344	phage late control D family protein	-
D1605_RS01845	454457..455428	-	972	WP_011097609.1	hypothetical protein	-
D1605_RS01850	455436..455993	-	558	WP_011097610.1	phage tail protein I	-
D1605_RS01855	455986..456879	-	894	WP_011097611.1	baseplate J/gp47 family protein	-
D1605_RS01860	456879..457217	-	339	WP_004090694.1	GPW/gp25 family protein	-
D1605_RS01865	457415..457696	+	282	WP_004090695.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1605_RS01870	457707..457982	+	276	WP_004085172.1	HigA family addiction module antidote protein	Antitoxin
D1605_RS01875	458070..458372	+	303	WP_004090696.1	type II toxin-antitoxin system MqsR family toxin	-
D1605_RS01880	458375..458776	+	402	WP_004090697.1	type II toxin-antitoxin system MqsA family antitoxin	-
D1605_RS01885	458845..459432	-	588	WP_011097612.1	phage baseplate assembly protein V	-
D1605_RS01890	459429..459971	-	543	WP_011097613.1	hypothetical protein	-
D1605_RS01895	459947..460468	-	522	WP_011097614.1	hypothetical protein	-
D1605_RS01900	460465..460788	-	324	WP_011097935.1	hypothetical protein	-
D1605_RS01905	460788..461048	-	261	WP_011097616.1	hypothetical protein	-
D1605_RS01910	461066..462940	-	1875	WP_011097617.1	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	446013..468846	22833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10639.21 Da
• Isoelectric Point: 10.0980

>T145529 WP_004090695.1 NZ_CP047171:457415-457696 [Xylella fastidiosa subsp. fastidiosa]
MIKSFRHKGIQQFFLKGSTAGIQTKHAAKLRIQLTALESAKRPEDMNAPGWKLHPLKGADLKGHWSIWVNGNYRLTFAFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T145529 NZ_CP062402:383068-383385 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10072.68 Da
• Isoelectric Point: 10.2184

>AT145529 WP_004085172.1 NZ_CP047171:457707-457982 [Xylella fastidiosa subsp. fastidiosa]
MKRMHNPAHPGEVLREYLGELTVTNAAAKLGVSRVALSRILNGTNGISADMALRLEDALGTSAEMWAAMQFKYDLWVASQ
QPRPKITRLHV

Nucleotide

• Length: 276 bp

>AT145529 NZ_CP062402:382856-383065 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: Q87EE4