Detailed information of TA system TA145523

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 126144..126397
• Replicon: plasmid pKP19-2029-KPC2
• Accession: NZ_CP047161
• Organism: Klebsiella pneumoniae strain KP19-2029

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: GSU00_RS27750
• Protein ID: WP_001312851.1
• Coordinates: 126248..126397 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	126144..126203 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSU00_RS27710	121804..121869	-	66	Protein_160	helix-turn-helix domain-containing protein	-
GSU00_RS27715	121922..122626	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
GSU00_RS27720	122690..122851	+	162	Protein_162	DNA helicase	-
GSU00_RS27725	122871..123617	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
GSU00_RS27730	123672..124232	+	561	WP_000139328.1	fertility inhibition protein FinO	-
GSU00_RS27735	124364..124564	+	201	WP_015059022.1	hypothetical protein	-
GSU00_RS27740	124950..125549	+	600	WP_032083981.1	hypothetical protein	-
GSU00_RS27745	125713..125943	+	231	WP_001736714.1	hypothetical protein	-
-	126144..126203	-	60	NuclAT_1	-	Antitoxin
-	126144..126203	-	60	NuclAT_1	-	Antitoxin
-	126144..126203	-	60	NuclAT_1	-	Antitoxin
-	126144..126203	-	60	NuclAT_1	-	Antitoxin
GSU00_RS27750	126248..126397	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
GSU00_RS27755	126681..126929	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / blaSHV-12 / blaKPC-2	-	1..127240	127240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T145523 WP_001312851.1 NZ_CP047161:126248-126397 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T145523 NZ_CP062401:2353322-2353429 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT145523 NZ_CP047161:c126203-126144 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin