Detailed information of TA system TA145497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 138447..139720
• Replicon: chromosome
• Accession: NZ_CP047159
• Organism: Prevotella sp. Rep29

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: GRF55_RS00580
• Protein ID: WP_220369568.1
• Coordinates: 138447..139388 (-)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: -
• Locus tag: GRF55_RS00585
• Protein ID: WP_220368656.1
• Coordinates: 139388..139720 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRF55_RS00555	134491..134871	+	381	WP_220368651.1	hypothetical protein	-
GRF55_RS00560 (GRF55_00560)	134855..135670	+	816	WP_220368652.1	DUF6291 domain-containing protein	-
GRF55_RS00565 (GRF55_00565)	135660..136172	+	513	WP_220368653.1	hypothetical protein	-
GRF55_RS00570 (GRF55_00570)	136910..137242	+	333	WP_220368654.1	hypothetical protein	-
GRF55_RS00575 (GRF55_00575)	137226..138335	+	1110	WP_220368655.1	HigA family addiction module antitoxin	-
GRF55_RS00580 (GRF55_00580)	138447..139388	-	942	WP_220369568.1	HipA domain-containing protein	Toxin
GRF55_RS00585 (GRF55_00585)	139388..139720	-	333	WP_220368656.1	HipA N-terminal domain-containing protein	Antitoxin
GRF55_RS00590 (GRF55_00590)	139717..139920	-	204	WP_220368657.1	helix-turn-helix transcriptional regulator	-
GRF55_RS00595 (GRF55_00595)	139968..140183	-	216	WP_220368658.1	hypothetical protein	-
GRF55_RS00600 (GRF55_00600)	140180..141646	-	1467	WP_220368659.1	phage integrase SAM-like domain-containing protein	-
GRF55_RS00605 (GRF55_00605)	142481..142633	-	153	WP_220368660.1	hypothetical protein	-
GRF55_RS00610 (GRF55_00610)	142869..143705	-	837	WP_220368661.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-281)

Antitoxin

• HipA, N-terminal subdomain 1 (5-101)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35725.30 Da
• Isoelectric Point: 7.8380

>T145497 WP_220369568.1 NZ_CP047159:c139388-138447 [Prevotella sp. Rep29]
MCKCKCLYCYQPLEEGQKDFHPGCSRKFFGTKDVPSMEYRHEDLDSLAEQVIRAQTSLTGVQPKLSLNLSKHEGCSRLTI
VGLWGDYIFKPQTDSYPQLPENEDLTMHLAEAAKINVVPHSLIRLADGELGYITKRIDRTKNGEKIDMEDMCQLTLHPTE
YKYKGSHEQIAKTIAQYSSTPKLDLTNYMQLLLFCFVTGNNDMHLKNFSLYRPSEKYQLTPAYDLLNVAIANPKDKEELA
LPLSGRKTKLRLDDFLKAAKTMGLEENVVLRLIAGLHKALPKWQALIQASFLCEDMKSAYLELVTSRLARLQR

Nucleotide

• Length: 942 bp

>T145497 NZ_CP062398:c1877982-1877830 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12287.18 Da
• Isoelectric Point: 4.0480

>AT145497 WP_220368656.1 NZ_CP047159:c139720-139388 [Prevotella sp. Rep29]
MRQAEIYRKDILAGILTEEGGEYRFCYDEAYLAREDAQPVSLTLPLQTEAFVSPVLFPFFDGLIPEGWLLDVALRNTDIS
VLDRMSLLLLCCKECIGSVSVVPINKEGGE

Nucleotide

• Length: 333 bp

>AT145497 NZ_CP062398:c1878606-1878007 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA